Regulatory

Part:BBa_J100531

Designed by: Kaitlin Cvijanovich, Alexandra Johansen, Yusef Thomas, Douglas Deutsch   Group: Campbell M Lab   (2020-02-06)


A constitutive promoter that has the tet-kil gene but not the cI857 repressor

The tet-kil is information placed between the -10 and -35 ends in a lacO promoter. Tet-Kil is lethal in E. coli so when induced it will kill the host cell which occurs at 42 degrees Celcius. Temperature dictates the efficiency of the kil gene. The promoter is regulatory.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None